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The Protein Protocols Handbook - Walker, John M.
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The Protein Protocols Handbook - neues Buch

ISBN: 9780896039407

The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide… Mehr…

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Walker, John M.:

The Protein Protocols Handbook (Methods in Molecular Biology) - gebunden oder broschiert

ISBN: 9780896039407

Humana Press. Hardcover. GOOD. Spine creases, wear to binding and pages from reading. May contain limited notes, underlining or highlighting that does affect the text. Possible ex libra… Mehr…

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Walker, John M.:
The Protein Protocols Handbook (Methods in Molecular Biology) - gebunden oder broschiert

ISBN: 9780896039407

Humana Press. Hardcover. VERY GOOD. Light rubbing wear to cover, spine and page edges. Very minimal writing or notations in margins not affecting the text. Possible clean ex-library cop… Mehr…

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John M. Walker (Editor):
Protein Protocols Handbook (Methods in Molecular Biology) - gebunden oder broschiert

2002, ISBN: 9780896039407

Hardcover, New book, Buy with confidence. Excellent Customer Service & Return policy. Ships Fast. 24*7 Customer Service., [PU: Humana Press]

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Editor-John M. Walker:
Protein Protocols Handbook (Methods in Molecular Biology) - gebunden oder broschiert

2002, ISBN: 9780896039407

Humana Press, 2002-04-15. Hardcover. Good., Humana Press, 2002-04-15

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Details zum Buch
The Protein Protocols Handbook

This greatly enhanced second edition introduces 60 critically important new chapters dealing with topics and techniques that have emerged since the first edition, as well as significantly updating the remaining articles. The new chapters cover many rapidly developing areas, particularly the application of mass spectrometry in protein characterization, as well as the now well-established 2-D PAGE technique in proteomics. The section on glycoprotein analysis has also been significantly expanded, and methods for the production of single-chain and phage-displayed antibodies have been added to the section on antibodies. Each readily reproducible method follows the highly praised format of the Methods in Molecular Biologya"( series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls and on modifying the method to function within your own experimental circumstances.

Detailangaben zum Buch - The Protein Protocols Handbook


EAN (ISBN-13): 9780896039407
ISBN (ISBN-10): 0896039404
Gebundene Ausgabe
Erscheinungsjahr: 2002
Herausgeber: Springer

Buch in der Datenbank seit 2009-01-14T01:09:34+01:00 (Berlin)
Detailseite zuletzt geändert am 2023-01-26T13:35:13+01:00 (Berlin)
ISBN/EAN: 0896039404

ISBN - alternative Schreibweisen:
0-89603-940-4, 978-0-89603-940-7
Alternative Schreibweisen und verwandte Suchbegriffe:
Autor des Buches: walker john
Titel des Buches: protein protocols


Daten vom Verlag:

Autor/in: John M. Walker
Titel: The Protein Protocols Handbook
Verlag: Humana Press; Humana Press
1146 Seiten
Erscheinungsjahr: 2002-02-15
Totowa; NJ; US
Gewicht: 0,004 kg
Sprache: Englisch
85,59 € (DE)
87,99 € (AT)
106,60 CHF (CH)
Not available, publisher indicates OP

BB; Book; Hardcover, Softcover / Biologie/Biochemie, Biophysik; Biochemie; Verstehen; protein; chemistry; molecular biology; proteins; biology; nucleic acid; C; Biochemistry, general; Biomedical and Life Sciences; BB; EA; BB

Part I. Quantitation of Proteins 1 Protein Determination by UV Absorption Alastair Aitken and Michèle P. Learmonth 2 The Lowry Method for Protein Quantitation Jakob H. Waterborg 3 The Bicinchoninic Acid (BCA) Assay for Protein Quantitation John M. Walker 4 The Bradford Method for Protein Quantitation Nicholas J. Kruger 5 Ultrafast Protein Determinations Using Microwave Enhancement Robert E. Akins and Rocky S. Tuan 6 The Nitric Acid Method for Protein Estimation in Biological Samples Scott A. Boerner, Yean Kit Lee, Scott H. Kaufmann, and Keith C. Bible 7 Quantitation of Tryptophan in Proteins Alastair Aitken and Michèle P. Learmonth 8 Flow Cytometric Quantitation of Cellular Proteins Thomas D. Friedrich, F. Andrew Ray, Judith A. Laffin, and John M. Lehman 9 Kinetic Silver Staining of Proteins Douglas D. Root and Kuan Wang Part II. Electrophoresis of Proteins and Peptides and Detection in Gels 10 Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins John M. Walker 11 SDS Polyacrylamide Gel Electrophoresis of Proteins John M. Walker 12 Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins John M. Walker 13 SDS-Polyacrylamide Gel Electrophoresis of Peptides Ralph C. Judd 14 Identification of Nucleic Acid Binding Proteins Using Nondenaturing Sodium Decyl Sulfate Polyacrylamide Gel Electrophoresis (SDecS-Page) Robert E. Akins and Rocky S. Tuan 15 Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins: Mr-Based Separation of Proteins with Retained Native Activity Robert E. Akins and Rocky S. Tuan 16 Acetic-Acid-Urea Polyacrylamide Gel Electrophoresis of Basic Proteins Jakob H. Waterborg 17 Acid-Urea-Triton Polyacrylamide Gel Electrophoresis of Histones Jakob H. Waterborg 18 Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels John M.Walker 19 Protein Solubility in Two-Dimensional Electrophoresis: Basic Principles and Issues Thierry Rabilloud 20 Preparation of Protein Samples from Mouse and Human Tissues for 2-D Electrophoresis Joachim Klose 21 Radiolabeling of Eukaryotic Cells and Subsequent Preparation for 2-D Electrophoresis Nick Bizios 22 Two-Dimensional Polyacrylamide Gel Electrophoresis Using Carrier Ampholyte pH Gradients in the First Dimension Patricia Gravel 23 Casting Immobilized pH Gradients (IPGs) Elisabetta Gianazza 24 Nonequilibrium pH Gel Electrophoresis (NEPHGE) Mary F. Lopez 25 Difference Gel Electrophoresis Mustafa Ünlü and Jonathan Minden 26 Comparing 2-D Electrophoretic Gels Across Internet Databases Peter F. Lemkin and Gregory C. Thornwall 27 Immunoblotting of 2-D Electrophoresis Separated Proteins Barbara Magi, Luca Bini, Sabrina Liberatori, Roberto Raggiaschi, and Vitaliano Pallini 28 Quantification of Radiolabeled Proteins in Polyacrylamide Gels Wayne R. Springer 29 Quantification of Proteins on Polyacrylamide Gels Bryan John Smith 30 Rapid and Sensitive Staining of Unfixed Proteins in Polyacrylamide Gels with Nile Red Joan-Ramon Daban, Salvador Bartolomé, Antonio Bermúdez, and F. Javier Alba 31 Zinc-Reverse Staining Technique Carlos Fernandez-Patron 32 Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels Jung-Kap Choi, Hee-Youn Hong, and Gyurng-Soo Yoo 33 Detection of Proteins in Polyacrylamide Gels by Silver Staining Michael J. Dunn 34 Protein Detection on Polyacrylamide Gels and Electroblots Using Transition Metal Chelate Stains Wayne F. Patton 35 Detection of Proteins in Polyacrylamide Gels by Fluorescent Staining Michael J. Dunn 36 Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using Eosin Y Stain Fan Lin and Gary E. Wise 37 Electroelution of

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